Generation of GM130 Conditional Knockout Mouse

Abstract

The Golgi apparatus is a common and highly dynamic organelle in eukaryotic cells. It plays an important role in secretory trafficking and cargo modifications. Increasing evidence suggests that structural changes and functional disorders of the Golgi apparatus are involved in many human diseases, but whether Golgi dysfunction is a causal factor in regard to the progression of these diseases remains unknown. GM130 has been postulated to play roles in Golgi stack formation and vesicular transport based on studies on cultured cells and in vitro reconstitutions. To define the role of GM130 in animal, a GM130 knockout mouse has recently been created. Based on the principle of homologous recombination, the GM130 conditional knockout mouse model was established through gene targeting, stem cell screening, and blastocyst injection. Such model has been successfully applied for studies of physiological functions of GM130 and Golgi apparatus at the cellular and animal levels.