Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.
CITATION STYLE
Ip, P. L., & Birnbaum, K. D. (2015). Tissue-specific gene expression profiling by cell sorting. In Plant Functional Genomics: Methods and Protocols: Second Edition (pp. 175–183). Springer New York. https://doi.org/10.1007/978-1-4939-2444-8_8
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