The composition of the culture medium influences the β-1,3- glucanmetabolism of Aspergillus fumigatus and the antifungal activity of inhibitors of β-1,3-glucan synthesis

Abstract

In vitro testing of Aspergillus fumigatus susceptibility to echinocandins has always been a challenge. Using a simple and quick colorimetric method to analyze the activity of inhibitors of β-1,3-glucan synthesis, we found that the composition of the culture medium significantly influences glucan synthesis and consequently the antifungal properties of inhibitors of &beta-1,3-glucan synthesis when they are tested alone or in combination with chitin synthase inhibitors. Copyright © 2012, American Society for Microbiology. All Rights Reserved.