STS markers for comparative mapping in legumes

Abstract

DNA primers for 37 genes have been developed in pea (Pisum sativum L.). Two-thirds of these primers also amplify orthologous sequences in lentil (Lens culinaris). The primers were designed to be complementary to highly conserved sequences in exons of known genes. In addition, most of the priming sequences were selected to be 1000 to 3000 bp distant on the genomic DNA and to amplify a fragment that contained at least one intron. Segregating sequence polymorphism in mapping populations of recombinant inbred lines (RILs) derived from wide crosses in Pisum was observed by restriction of the amplified fragment with endonucleases recognizing four-base restriction sites. Successful mapping of 36 of these genes in pea demonstrated the utility of these primers for mapping, and it appears likely that the primers should have general utility for comparative mapping in legumes.