Alginate oligomer induces nitric oxide (NO) production in RAW264.7 cells: Elucidation of the underlying intracellular signaling mechanism

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Abstract

Alginate is an acidic linear polysaccharide with immune-modulating activities. In this study, we found that enzymatically digested alginate oligomer (AO) with various degrees of polymerization (DP; 2-5) induced a higher level of nitric oxide (NO) production in RAW264.7 cells than undigested alginate polymer (AP). Reverse transcription-polymerase chain reaction and western blot analyses revealed that the expression level of inducible NO synthase in AO-treated RAW264.7 cells was higher than that in AP-treated cells. AO induced nuclear translocation of nuclear factor (NF)-κB p65 subunit in RAW264.7 cells to a greater extent than AP. Although AO and AP induced similar extents of phosphorylation in three mitogen-activated protein (MAP) kinases, c-Jun N-terminal kinase inhibitor exhibited the most potent inhibitory effect on NO induction in AO-and AP-treated RAW264.7 cells, among three MAP kinase inhibitors that were tested.

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Ueno, M., Cho, K., Nakazono, S., Isaka, S., Abu, R., Takeshita, S., … Oda, T. (2015). Alginate oligomer induces nitric oxide (NO) production in RAW264.7 cells: Elucidation of the underlying intracellular signaling mechanism. Bioscience, Biotechnology and Biochemistry, 79(11), 1787–1793. https://doi.org/10.1080/09168451.2015.1052768

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