Probiotic enriched Daphnia sp: The nutritional profile and enzymatic activities

Abstract

Live food played an important role in aquaculture especially hatcheries. This study was carried out to evaluate the effect of enriched different probiotic doses on total lenght, nutritional profile and enzymatic activities of Daphnia sp. This research used 3 treatments and 3 replications. The experimental treatments are 0, 5 and 10 ml L-1 and enriched period was 0, 12 and 24 hours. Probiotic consortia bacteria of Bacillus subtilis (107 CFU ml-1) and Lactobacillus casei (107 CFU ml-1) which is fermented by the addition of spices and molasses. Daphnia sp. was cultured in fresh water with a density of 20 ind ml-1. Observed parameters include total lenght, nutritional profile and enzymatic activities. The results showed that probiotic doses effect significantly different (p<0.05). In this research, the best performance enriched at 10 ml L-1 doses of probiotic and 12 hours incubation period included total lenght of 1.8 mm, and moisture, protein, lipid, ash amounted to 98.43±0.03, 42.72±0.3, 6.34±0.07, 11.72±0.39 %, protease and lipase activity were 21.92±0.90 U ml-1 and 0.82±0.06 U ml-1. Enriched Daphnia sp. has live food potential for hatcheries.