In vitro analysis of phytochemicals epicatechin, naringenin, myricetin against Hep3B cell lines and VERO cell lines

Abstract

Hepatocellular carcinoma ranks as the eighth most common cancer, sixth among men and eleventh among women and accounts for half a million deaths per year. Natural products, especially plants have been used for the treatment of various diseases for thousands of years which contain no side effects. The main aim and objective of the study is in vitro analysis of compounds such as epicatechin, myricetin, naringenin against Hep3B cell line and VERO cell line. In vitro assay of Hep3B cell line and VERO cell line was done by MTT assay. The assay is based on the reduction of soluble yellow tetrazolium salt to insoluble purple formation crystals by metabolically active cells. In vitro assay revealed that compounds such as epicatechin, myricetin and naringenin have efficacy in Hep3B cancer cell line and VERO normal cell line. By comparing the obtained results of MTT assay with these literature survey reports, it could be concluded that the compound has the potential to protect the cells from damage and the possible mechanism might be the inhibition of oxidative and inflammatory processes.