Isolation and antimicrobial activity of feruloyl oligosaccharide ester from pineapple stem residues

Abstract

Ferulic, ρ-coumaric and ρ-hydroxybenzoic acids, a major components of phenolic acid in pineapple stem were extracted by alkali-treatment. The total contents of these alkali extractable phenolic acids were estimated as 8.5, 15.3, and 8 mg/g of the plant tissues, respectively. Feruloyl oligosaccharide ester (FE) was released from this plant tissues by the treatment with the crude enzyme of Sporotrichum sp. HG 1. Synergism between purified carboxymethyl cellulase (CMCase) and xylanase of this strain was observed for the production of FE from this tissues. The FE was found to be more effective than ferulic acid monomer for bacterial growth. The FE showed inhibitory effect against many kinds of gram positive and negative bacteria except Lactobacillus delbrueckii, Streptococcus mutans, S. salivarius, Enterococcus faecalis, and E. casseliflavus.