Formulation And Evaluation Polyherbal Gel Against Staphylococcus Aureus Bacteria Causing Skin Disease

Abstract

Objective: Over past few years, herbal drugs have been gradually shown a revival of interest for the use of medicinal plants to be used against various disorders. Not limited to developing countries but developed areas are also inclined towards the use because herbal medicines as they have been reported to be safe with least adverse effects especially in comparison to the synthetic drugs. Aim of this research is to formulate and evaluate polyherbal gel against bacteria. Method: In the present study we have prepared a topical gel formulation  which is comprised of  combined drug aqueous extract of  Murraya Koeniggi, Eucalyptus globulus, Dodonaea viscosa and Mentha spicata in a concentration of 19.2% respectively in a base. The base was prepared by using carbapol 940, propylene glycol-400, glycerine, honey, propylparaben, EDTA, triethanolamine and required amount of water in a quantity sufficient to prepare 100g. The prepared formulation were evaluated for its antimicrobial activity by agar well diffusion technique against S. aureus, Gram positive bacteria by using agar well diffusion method. Further, the formulation was also undergone physical evaluation for appearance and homogeneity, pH, viscosity and rheological studies, spreadability, skin irritation test (Patch test) and washability. Result: The results of the study revealed that the formulated gel has shown better zone of inhibition against staphylococcus aureus bacteria causing skin disease.   Conclusion: Based on our research, it could be concluded that this formulations possess a positive antimicrobial activity and can be used safely on human skin.