We recently reported polymorphism in the mitochondrial genes of Tribolium castaneum, a stored grain pest. Here, we show that T. castaneum samples from the main island of Japan can be easily differentiated from samples from other areas by using real-time PCR with minor groove binding (MGB) TaqMan probes containing a single nucleotide polymorphism (SNP) to amplify a 179-bp fragment. This assay was also applicable to samples stored at room temperature for 5 months after natural death, and samples whose DNA was artificially degraded by heat, pressure, or gamma-ray irradiation.
CITATION STYLE
Yamauchi, H., Harada, M., & Miyanoshita, A. (2018). Differentiation of laboratory strains of red flour beetle tribolium castaneum collected from various areas. Journal of the Food Hygienic Society of Japan, 59(5), 220–222. https://doi.org/10.3358/shokueishi.59.220
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