Evaluation of the Cornea Cells Affected by Multi-Purpose Solutions for Contact-Lens

Abstract

We studied the influence of multi-purpose solutions (MPS) ongap-junctional intercellular communication (GJIC) of cornea cells andits cell toxicity such as cell metabolism and proliferation. This studyevaluates long-term safety of three types of multi-purpose solutions(MPS) in the cornea cells. Three types of MPS were investigated usingscrape-loading dye transfer (SLDT) method to measure GJIC. Cell toxicitywas evaluated for cell viability according to colony method and USPelution method (MEM Elution Test). In the SLDT method, one type solutionwas significantly showed an inhibitory effect on the GJIC in corneacells but not the other type solutions. Comparative cytotoxicitypotentials of three types of MPS also indicated similar tendency in theresult of SLDT method. These results suggest that the evaluation methodsare considered to be effective to confirm the safety of MPS.