COMPARISON OF NEURONAL AND LENS PHENOTYPE EXPRESSION IN THE TRANSDIFFERENTIATING CULTURES OF NUERAL RETINA WITH DIFFERENT CULTURE MEDIA

Abstract

The effects of three different culture media (Eagle's MEM, F‐12 and L‐15) on the transdifferentiation of 8‐day chick embryonic neural retina into lens cells, were examined with respect to the expression of two phenotypes. One type referred to neuronal specificity (as represented by the level of cholineacetyl‐transferase, CAT, activity) and the other to lens specificity (as represented by content of α‐and δ‐crystallin). In 7‐day cell cultures before the visible differentiation of lentoid bodies, CAT activity was detected in all media. But, its level was about 9 times higher in cultures with L‐15 than in those with MEM and 3 times higher than in F‐12. In 26‐day cultures, CAT activity was practically undetectable. The production of α‐and δ‐crystallin was detected in cultures at 26 days. There were quantitative differences in the crystallin content with different media, and it was highest in cultures with L‐15. The results indicate that conditions most favourable to the maintenance of the neuronal specificity in cell cultures of neural retina, can also support the most extensive transdifferentiation. The possibility of direct transdifferentiation of once neuronally specified cells into lens cells in cultures with L‐15 has been suggested to explain the present results. Copyright © 1980, Wiley Blackwell. All rights reserved