In vitro polyploidy induction of foxtail millet (Setaria italica (L) beauv) cv. buru hotong using colchicine treatment

Abstract

In vitro polyploidy induction of foxtail millet (Setaria Italica (L.) Beauv) was done using callus from the basal leaf of 10-day old seedlings. Callus was treated using three different concentrations of colchicine (0, 125, 250 and 500 ppm) and two exposure times (24 and 48 hours). Results showed that colchicine declines the survival rate and the number of shots on all combinations. Putative polyploidy plant was produced on the combination of K3T1 (500 ppm colchicine; 24 hour exposure time). Based on field experiment data, putative polyploid plant has smaller characteristics in the term of plant height, the number of tillers, and number of seed per panicle than the diploid plant. However, leaf width, panicle length and diameter, seed size, and weight of 100 seeds of putative polyploid plant bigger than the diploid plant.