Effect of some phytoestrogens on metabolism of rat adipocytes

Abstract

The aim of this experiment was to evaluate the direct effect of genistein, daidzein and zearalenone on basal and hormone-induced lipogenesis and lipolysis in isolated rat adipocytes. In lipogenesis, daidzein and zearalenone were used at concentrations of 0.01, 0.1 and 1 mmol·L-1 and genistein at concentrations of 0.01, 0.3, 0.6 and 1 mmol·L-1. In lipolysis, concentrations of tested compounds were 0.01, 0.1 and 1 mmol·L-1. All tested compounds clearly inhibited basal and insulin (1 nmol·L-1) stimulated lipogenesis. Basal lipolysis was particularly enhanced by genistein and daidzein at its higher concentrations. The ability of zearalenone to potentiate of basal lipolysis was less marked. Epinephrine (1 μmol·L-1)-stimulated lipolysis was inhibited by genistein at 1 mmol·L-1 At a concentration of 0.1 mmol·L-1 daidzein also augmented epinephrine-stimulated lipolysis and at its highest concentration exhibited an inhibitory effect, similar to genistein. Zearalenone reduced stimulated lipolysis, particularly at the highest concentration. (C) Inra/Elsevier, Paris.