RNA Isolation and Characterization Protocols

Abstract

Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols is the single best collection of easily reproducible, step-by-step RNA techniques now available for today's bench scientists. Introduction to isolating RNA / Donald E. Macfarlane, Christopher E. Dahle -- Large and small scale RNA preparations from eukaryotic cells / Wolfgang Uckert, Wolfgang Walther, Ulrike Stein -- An improved rapid method of isolating RNA from cultured cells / David B. Batt, Gordon G. Carmichael, Zhong Liu -- Isolating RNA with the cationic surfactant, Catrimox-14 / Christopher E. Dahle, Donald E. Macfarlane -- RNA extraction from formalin-fixed and paraffin-embedded tissues / Giorgio Stanta, Serena Bonin, Rosella Perin -- Extraction and purification of RNA from plant tissue enriched in polysaccharides / Shu-Hua Cheng, Jeffrey R. Seemann -- Isolation of plant mitochondrial RNA from green leaves / Fei Ye, Wolfgang O. Abel, Ralf Reski -- Extraction of RNA from fresh and frozen blood / Bimal D. Theophilus -- Isolation of total RNA from bacteria / John Heptinstall -- Isolation of total RNA from tissues or cell lines : visualization in gel / Tapas Mukhopadhyay, Jack A. Roth -- Isolation of messenger RNA / Sian Bryant, David L. Manning -- UV spectrophotometric analysis of ribonucleic acids / Ralph Rapley, John Heptinstall -- Formaldehyde gel electrophoresis of total RNA / Sian Bryant, David L. Manning -- Preparation of RNA dot-blots / Rachel Hodge -- Nonradioactive Northern blotting / Rainer Löw -- The use of RNA probes for the analysis of gene expression : Northern blot hybridization and ribonuclease protection assay / Dominique Belin -- Analysis of RNA by Northern blotting using riboprobes / Rai Ajit Srivastava -- RNA quantitative analysis from fixed and paraffin-embedded tissues / Giorgio Stanta, Serena Bonin, Renè Utrera -- Quantitative analysis of RNA species by PCR and solid-phase minisequencing / Anu Suomalainen, Ann-Christine Syvänen -- Preparation of tissue sections and slides for mRNA hybridization / Giorgio Terenghi -- Detecting mRNA in tissue sections with digoxigenin-labeled probes / Giorgio Terenghi -- One-tube RT-PCR with sequence-specific primers / Ulrich Pfeffer -- Identification of differentially expressed genes by nonradioactive differential display of messenger RNA / Thomas C. Bosch, Jan U. Lohmann -- Characterization of RNA using continuous RT-PCR coupled with ELOSA / François Mallet, Guy Oriol, Bernard Mandrand -- Gene expression analysis by CD-RT-PCR / Eric de Kant -- Primer extension analysis of mRNA / Maggie Walmsley, Mark Leonard, Roger Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Measurements of rate of transcription in isolated nuclei by nuclear "run-off" assay / Rai Ajit Srivastava, Gustav Schonfeld -- Transcription in vitro using bacteriophage RNA polymerases / Elaine T. Schenborn -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- The Xenopus egg extract translation system / Glenn M. Matthews, Alan Colman -- Purification and characterization of viral dsRNA genome profiles by crosshybridization / Lesley-Ann Martin, Peter P. Mertens.