Post-Column Fluorometric Detection Of Reducing Sugars In High Performance Liquid Chromatography Using Arginine

Abstract

Arginine was found to react with reducing sugars after being heated in boric acid solution at neutral pH; highly fluorescent derivatives were produced. This reaction was applied to a postcolumn reaction system for high performance liquid chromatography and found to be useful for highly sensitive determination of reducing sugars. © 1983, The Japan Society for Analytical Chemistry. All rights reserved.