Estimation of the enzymes LDH, GOT and GPT in plasma of grey mullet Mugil auratus and their significance in liver intoxication

Abstract

Activities of lactic dehydrogenase (LDH), glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were measured in blood plasma and some tissues of grey mullet Mugil auratus Risso. After determination of optimal assay conditions, cardiac sampling for LDH estimation was recommended. Tissue distribution of enzymes and their origin in plasma were described, in particular the isozyme forms of LDH. Plasma levels of all 3 enzymes showed a significant increase following treatment with CCl sub(4).