Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.
CITATION STYLE
Oh, Y., Lee, W. jae, Hur, J. K., Song, W. J., Lee, Y., Kim, H., … Lee, S. H. (2022). Expansion of the prime editing modality with Cas9 from Francisella novicida. Genome Biology, 23(1). https://doi.org/10.1186/s13059-022-02644-8
Mendeley helps you to discover research relevant for your work.