Nutritionally-directed compensatory growth enhances mammary development and lactation potential in rats

Abstract

A nutritionally-regulated compensatory growth regimen imposed during a growing period from prepuberty to gestation can significantly affect mammary development and subsequent lactation performance. The objectives of this study were as follows: 1) to determine whether a compensatory nutrition regimen enhances lactation potential for the first and second lactation cycles and 2) to determine the extent to which a compensatory nutrition regimen modulates cell proliferation, differentiation, and apoptosis and expression of genes in mammary tissues of female rats. Female Sprague-Dawley rats (n = 122, 35 d of age) were randomly assigned either to the control group, with free access to diet, or to a stair-step compensatory nutrition feeding regimen, with an alternating 2-2-3-3-wk schedule. The regimen began with an energy-restricted diet (40% restriction) for 2 wk, followed by the control diet for 2 wk; this step was then repeated at 3-wk intervals. Pups of dams from the compensatory nutrition regimen group gained more during mid- lactation than did control group pups. Mammary tissues were obtained from early (d 2) and late (d 19) lactating rats. Mammary tissue from the compensatory nutrition group exhibited increased cell proliferation and greater γ-glutamyltranspeptidase and ornithine decarboxylase gene expressions than did tissue from the control group during early lactation of both cycles. Mammary tissue from the compensatory nutrition group also had fewer apoptotic cells than tissue from the control group during late lactation of the first lactation cycle. These results suggest that the compensatory nutrition regimen imposed during the peripubertal developmental phase stimulated mammary growth and enhanced lactation performance by affecting the expression of genes that regulate the cell cycle.