Regulator of G Protein Signaling 8 (RGS8) Requires Its NH2 Terminus for Subcellular Localization and Acute Desensitization of G Protein-gated K+ Channels

Abstract

Functional roles of the NH2-terminal region of RGS (regulators of G protein signaling) 8 in G protein signaling were studied. The deletion of the NH2-terminal region of RGS8 (ΔNRGS8) resulted in a partial loss of the inhibitory function in pheromone response of yeasts, although Gα binding was not affected. To examine roles in subcellular distribution, we coexpressed two fusion proteins of RGS8-RFP and ΔNRGS8-GFP in DDT1MF2 cells. RGS8-RFP was highly concentrated in nuclei of unstimulated cells. Coexpression of constitutively active Gαo resulted in translocation of RGS8 protein to the plasma membrane. In contrast, ΔNRGS8-GFP was distributed diffusely through the cytoplasm in the presence or absence of active Gαo. When coexpressed with G protein-gated inwardly rectifying K+ channels, ΔNRGS8 accelerated both turning on and off similar to RGS8. Acute desensitization of G protein-gated inwardly rectifying K+ current observed in the presence of RGS8, however, was not induced by ΔNRGS8. Thus, we, for the first time, showed that the NH2 terminus of RGS8 contributes to the subcellular localization and to the desensitization of the G protein-coupled response.