Main laboratory methods used for the isolation and identification of Staphylococcus spp.

Abstract

Introduction: Laboratory diagnosis related to clinical microbiology is highly complex, due to subjectivity when interpreting the results. Among bacterial pathogens, those linked to the Staphylococcus genus are relevant from a clinical and epidemiological the literature relative to the main laboratory methods used for the isolation and identification of microorganisms of the Staphylococcus. Methodology: The study was based on a literature search between 2000 and 2020, in the BVS, PubMed, Scielo, ScienceDirect and Periodical databases. Regarding its laboratory diagnosis, microscopy is used by the Gram method to verify the bacterium’s morphology; novobiocin test, aiming trace coagulase negative strains; use of biochemical tests, such as coagulase and catalase tests; identification through the use of selective and nonspecific culture media, such as blood agar (in which the hemolytic patterns of pathogens are observed), species of Staphylococcus aureus) and DNAse agar (selective to S. aureus strains, S. intermedius and S. hyicus). Results: The Staphylococcus in the field of public health. Thus, the quality and speed of diagnosis are essential to promote measures to combat these contributing to the well-being and survival of those affected.