Calcium sensors are indispensable tools to study the role of Ca2+ and visualize Ca2+ dynamics during biological processes. Over the past years, the field of Ca2+ imaging has strongly expanded by the development of a wide palette of sensors and optimization of sample handling. Here, we provide guidelines for imaging of the Ca2+ sensor R-GECO1 in Arabidopsis thaliana roots which can be interpolated to other intensiometric Ca2+ sensors. Furthermore, we demonstrate a procedure for image analysis of the acquired time-lapse recordings.
CITATION STYLE
Himschoot, E., Krebs, M., Costa, A., Beeckman, T., & Vanneste, S. (2018). Calcium ion dynamics in roots: Imaging and analysis. In Methods in Molecular Biology (Vol. 1761, pp. 115–130). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7747-5_9
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