Bacteriolyses of Bacterial Cell Walls by Cu(II) and Zn(II) Ions Based on Antibacterial Results of Dilution Medium Method and Halo Antibacterial Test

Abstract

Bacteriolyses of bacterial cell walls by copper (II) ions and zinc (II) ions based antibacterial results of broth dilution medium method and halo antibacterial test were investigated. From dilution medium method, MIC=625mg/L, MBC=1250mg/L for Cu 2+ solution as bactericide action were obtained against Staphylococcus aureus, and also from halo antibacterial test, the high antibacterial effects for Cu 2+ , Zn 2+ ions were obtained against Staphylococcus epidermidis. Bacteriolysis of S. aureus peptidoglycan (PGN) cell wall by Cu 2+ ions is ascribed to the inhibition of PGN elongation due to the damages of PGN biosyntheses TG, TP and the activations of PGN autolysins. The other, bacteriolysis of E. coli outer membrane cell wall by Cu 2+ ions is attributed tothe destruction of outer membrane structure and to the inhibition of PGN elongation due to the damage of PGN biosynthesis TP and the activations of PGN autolysins. Furthermore, bacteriolysis of S. aureus PGN cell wall by Zn 2+ ion is due to the inhibition of PGN elongation owing to the activations of PGN autolysins of amidases. The other, bacteriolysis of E. coli cell wall by Zn 2+ ions is attributed to the destruction of outer membrane structure due to degradative enzymes of lipoproteins at N-, and C-terminals, whereas is dependent on the activities of PGN hydrolases and autolysins of amidase and carboxy peptidase-transpeptidase. Cu 2+ and Zn 2+ ions induced ROS such as O 2 -, H 2 O 2 , • OH, OH-producing in bacterial cell wall occur oxidative stress. Keywords: MIC, MBC, CFU measurements and Halo antibacterial test, Cu 2+ and Zn 2+ ions, PGN cell wall, Outer membrane lipoproteins, Biosynthesis and autolysin, Reactive oxygen species(ROS).