Protein prenylation is required for membrane anchorage of small GTPases. Correct membrane targeting is essential for their biological activity. Signal output of the prenylated proto-oncogene Ras in addition critically depends on its organization into nanoscale proteolipid assemblies of the plasma membrane, so called nanoclusters. While protein prenylation is an established drug target, only a handful of nanoclustering inhibitors are known, partially due to the lack of appropriate assays to screen for such compounds. Here, we describe three cell-based high-throughput screening amenable Förster resonance energy transfer NANOclustering and Prenylation Sensors (NANOPS) that are specific for Ras, Rho, and Rab proteins. Rab-NANOPS provides the first evidence for nanoclustering of Rab proteins. Using NANOPS in a cell-based chemical screen, we now identify macrotetrolides, known ionophoric antibiotics, as submicromolar disruptors of Ras nanoclustering and MAPK signaling. © 2012 Elsevier Ltd All rights reserved.
CITATION STYLE
Köhnke, M., Schmitt, S., Ariotti, N., Piggott, A. M., Parton, R. G., Lacey, E., … Abankwa, D. (2012). Design and application of in vivo FRET biosensors to identify protein prenylation and nanoclustering inhibitors. Chemistry and Biology, 19(7), 866–874. https://doi.org/10.1016/j.chembiol.2012.05.019
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