Characterization of Bioactive compound isolated from Myrothecium spp. with UV, FTIR and HPLC Analysis

Abstract

Development of new drugs, especially in area of infectious diseases, represents today one of the most important research. Fungal isolates are receiving increasing attention by natural product chemists due to their diverse and structurally unprecedented compounds making them interesting candidates for drug discovery. In fact, need for novel, safe and more efficient antibiotics is a key challenge to the pharmaceutical industry today, moreover, increase in opportunistic infections in the immune compromised host has influenced this demand. Nowadays, evaluating morphological and biochemical differences as well as studying fungal genetic diversity via molecular indicators seem to be the most common method for screening this genus. In this research we evaluate the potential of bioactive compound, production and characterize the UV and FTIR spectroscopy and HPLC (High performance liquid chromatography) analysis pattern of isolated Myrothecium spp. MRP001. Process development for high level production of bioactive compound was applied using OFAT method. Then, following the extraction of secondary metabolite, the UV and FTIR spectroscopy analysis was carried out for characterization of the various extracts. Considering the coordinate analysis of UV and FTIR spectroscopy pattern, the isolate MRP001 with substantial antimicrobial activity exhibited absorption at 3411 cm-1 which is indicator of hydroxyl groups, absorption at 2856 and 2915 cm-1 indicating hydrocarbon chassis, and absorption at 1649 cm-1 indicating a double bond of polygenic compound. These results highlight the importance of Myrothecium isolates in antibiotic production. HPLC confirmed the production when compared with standards