The design and synthesis of novel genes and deoxyribonucleic acid (DNA) sequences is a central technique in synthetic biology. Current methods of high throughput gene synthesis use pooled oligonucleotides obtained from custom-designed DNA microarray chips, and rely on orthogonal (non-interacting) polymerase chain reaction primers to specifically de-multiplex, by amplification, the precise subset of oligonucleotides necessary to assemble a full length gene. The availability of a large validated set of mutually orthogonal primers is therefore a crucial reagent for high-throughput gene synthesis. Here, we present a set of 166 20-nucleotide primers that are experimentally verified to be non-interacting, capable of specifying 13 695 unique genes. These primers represent a valuable resource to the synthetic biology community for specifying genetic components that can be assembled through a scalable and modular architecture.
CITATION STYLE
Subramanian, S. K., Russ, W. P., & Ranganathan, R. (2018). A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components. Synthetic Biology, 3(1). https://doi.org/10.1093/synbio/ysx008
Mendeley helps you to discover research relevant for your work.