Photosystem II-Based Biosensors for Phytoremediation

Abstract

Using biosensors to det. herbicide action sites at the D1 protein level was studied. Two devices were built to analyze herbicide resistance. The first was composed of several vials in series with plant petioles kept in bubbled solns. contg. the herbicide under light. The second biosensor used a sensor which provided excited light and detected the fluorescent signal emitted by a biomediator set in a chamber where herbicide soln. is allowed to flow. Results showed the binding of herbicides to thylakoids. A redn. of the fluorescence induction area was not obsd. for a Poa annua mutant, but was obsd. for a Amaranthus retroflexus mutant. The use of 2 biosensors allowed the choice of photosynthetic plants suitable for phytoremediation and avoided laborious field plant testing. [on SciFinder(R)]