Catechin-7-O-β-D-glucopyranoside (CA-G) was previously isolated from red bean (the seed of Phaseolus calcaratus cv. Roxburgh). This study examined the ability of CA-G to scavenge reactive oxygen species generated by cell-free systems and to protect cells from oxidative stress caused by hydrogen peroxide (H2O2). The mechanism by which CA-G exerts its antioxidant and anti-apoptotic action on H2O2-exposed cells was also investigated. CA-G treatment prevented H2O2-mediated apoptosis and inhibited the formation of single stand breaks in DNA in H2O2- exposed BJAB cells. CA-G suppressed mitochondrial stress and caspase activation caused by H2O2. Mechanistic experiments revealed that the antioxidant mechanism of CA-G on H2O2-mediated oxidative damage was due to the direct scavenging of hydroxyl radicals and/or to the chelation of metal ions that were used to produce hydroxyl radicals from H2O2 via the Fenton reaction. Collectively, these findings suggest beneficial roles of CA-G or CA-Grich red bean on the protection from oxidative damage. © KoSFoST and Springer 2011.
CITATION STYLE
Baek, J. A., Fang, M., Lee, J. C., Son, Y. O., Lee, Y. J., Cho, H. K., … Whang, W. K. (2011). Catechin-7-O-β-D-glucopyranoside scavenges free radicals and protects Human B lymphoma BJAB Cells on H2O2-mediated oxidative stress. Food Science and Biotechnology, 20(1), 151–158. https://doi.org/10.1007/s10068-011-0021-x
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