The chemical synapse displays specialized intercellular adhesion between pre- and potsynaptic plasma membranes mediated by synaptic cell adhesion proteins. In this asymmetric cell adhesion, pre- and postsynapses have their own unique functions; the presynaptic terminal releases neurotransmitter, which diffuses through the synaptic cleft and is received by receptors accumulated at the postsynapse. Such distinct modes of actions of pre- and postsynapses in synaptic neurotransmission are the rate-limiting factors in signal processing in the brain, and thus protein-protein interactions within the pre- and postsynaptic scaffold are of particular importance for brain function by regulating the pre- and postsynaptic function. In the present paper, we outline a method to screen for binding partners of synaptic scaffold proteins biochemically.
CITATION STYLE
Altas, B., Jahn, O., & Kawabe, H. (2017). Biochemical purification of binding partners of synaptic scaffold proteins. In Methods in Molecular Biology (Vol. 1538, pp. 69–82). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6688-2_6
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