A simple extractive technique for honey flavonoid HPLC analysis

Abstract

A simple technique for routine analysis of flavonoids from honey has been described applied to the analysis of flavonoids in 27 honey samples from the La Alcarria region (Spain). The to- tal flavonoid content of the different samples ranged between 5 and 20 μg flavonoid/g honey. The utilising a combination of filtration through the resin Amberlite XAD-2 and extraction with ethyl ether. The proposed method is less complex than other methods for honey flavonoid analysis reported pre- viously. The HPLC conditions for flavonoid analysis have also been improved. This technique was major flavonoids in these samples were the flavanones pinocembrin and pinobanksin and the fla- vone chrysin. A total of 18 different flavonoids were detected in the honey samples analysed.