Much information can be obtained from knowledge of the relative expression level of each gene in the transcriptome. With the current advances in technology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10(6)-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization to DNA microarrays.
CITATION STYLE
Stangegaard, M. (2009). RNA preparation and characterization for gene expression studies. Methods in Molecular Biology (Clifton, N.J.), 529, 115–132. https://doi.org/10.1007/978-1-59745-538-1_8
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