Antimicrobial activity of selected wild mushrooms from different areas of bosnia and herzegovina

Abstract

The aim of this study was to examine the antimicrobial activity of selected wild mushrooms from different areas of Bosnia traditionally used in the diet. These are the first tests of antimicrobial activity of fungi extracts growing in Bosnia and Herzegovina. Wild mushrooms Boletus edulis Bull. (1782) and Cantharellus cibarius Fr. (1821) were collected from different areas of Bosnia and Herzegovina. Nine microbial strains were tested: four Gram-positive bacteria (Staphylococcus aureus ATCC 25923; Methicillin-resistant Staphylococcus aureus: MRSA ATCC 33591; Bacillus subtilis ATCC 6633; and Enterococcus faecalis ATCC 29212), four Gram-negative bacteria (Salmonella abony ATCC 6017; Pseudomonas aeruginosa ATCC 9027; Escherichia coli ATCC 25922; Extended Spectrum Beta-Lactamase producing E. coli: ESBL E. coli ATCC 35218), and one yeast Candida albicans ATCC 1023, through the agar well diffusion method. Investigated wild mushrooms performed antibacterial activity against three strains of Gram-positive bacteria: S. aureus, MRSA and B. subtilis. Antibacterial activity of the examined extract depends on the locality on which mushrooms grow. The broader spectrum of antibacterial activity was observed in case of C. cibarius extracts, and largest inhibition zones with this mushroom species were recorded against S. aureus (18.11 ± 0.20 mm) and B. subtilis (18.10 ± 0.17 mm). Extracts of B. edulis exhibited the largest inhibition zones against MRSA (20.03 ± 0.08 mm). In comparison to the standard antibiotic, tested extracts showed significant inhibition of this multidrug-resistant pathogen.