Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states. ©2007 European Molecular Biology Organization.
CITATION STYLE
Kim, H. J., Seol, J. H., Han, J. W., Youn, H. D., & Cho, E. J. (2007). Histone chaperones regulate histone exchange during transcription. EMBO Journal, 26(21), 4467–4474. https://doi.org/10.1038/sj.emboj.7601870
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