Bacterial antigen detection in body fluids: Methods for rapid antigen concentration and reduction of nonspecific reactions

Abstract

The authors sought procedures which would allow a rapid concentration in high yield of bacterial antigens from tissue fluids of patients and which could be applied also to protein-rich fluids like serum. Ethanol precipitation at a subzero temperature with albumin added as an antigen coprecipitant made it possible to achieve a more than 20-fold concentration of antigen in 15 min and a 200-fold concentration in 45 min. Heat-stable antigens could be concentrated from protein-rich fluids (like serum) after the sample had been deproteinized by boiling. Such heating (100°C, 3 min) also liberated bacterial polysaccharides from antibody complexes and eliminated the nonspecific interferences of serum in enzyme-linked immunosorbent assay.