The allosteric role of the AAA+ domain of ChlD protein from the magnesium chelatase of synechocystis species PCC 6803

Abstract

Background:Magnesium chelatase catalyzes the first essential step in chlorophyll biosynthesis. Results:Mutations in the AAA+ domain of the magnesium chelatase ChlD subunit reduce but do not abolish catalytic activity. Conclusion:ChlD is an allosteric regulator of magnesium chelatase. Significance:These observations reveal an essential role for the ChlD protein in the first committed stage in chlorophyll biosynthesis. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.