Identification, biodegradation and bio-evaluation of biopolymer produced from Bacillus thuringenesis

Abstract

Poly3-hydroxybutyric acid (PHB) is a promising eco-friendly substance favorable for medical use. PHB was produced from local isolate Bacillus thuringienesis in a conc. 4.1 g/L using 30 g/L Sugar-cane molasses (SCM) at pH 7.5 and incubation temperature 35°C. PHB biodegradation by soil microorganisms were completed after four weeks. Cell cytotoxicity testing is one of the critical factors affecting the biomedical application of polymers. 50% cell cytotoxic concentration (IC50) = 130 mg/mL while non-toxic concentration was 12.5 mg/mL studying of direct contact application between biopolymer and peripheral blood lymphocytes resulting monomers have no toxic effect on the cells.