Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cellautonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80% with up to 80 grafts assembled per hour can be achieved.
CITATION STYLE
Melnyk, C. W. (2017). Grafting with arabidopsis thaliana. In Methods in Molecular Biology (Vol. 1497, pp. 9–18). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6469-7_2
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