Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.
CITATION STYLE
Han, W., & Li, L. (2018). Chemical isotope labeling LC-MS for human blood metabolome analysis. In Methods in Molecular Biology (Vol. 1730, pp. 213–225). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7592-1_14
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