Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Vordermeier, M., & Whelan, A. O. (2012). ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis. Methods in Molecular Biology, 792, 219–227. https://doi.org/10.1007/978-1-61779-325-7_17
Mendeley helps you to discover research relevant for your work.