Abstract
This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.
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CITATION STYLE
Zhang, W., Watt, S. M., & Roberts, D. J. (2019). An innovative standard operation procedure for isolating GMP-Grade CD4+CD25+ T cells from non-mobilized leukapheresis. In Methods in Molecular Biology (Vol. 1899, pp. 67–83). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8938-6_6
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