Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify Drp1-related mitochondrial fission by labeling the mitochondrial import receptor subunit TOM20 in fixed cell culture.
CITATION STYLE
Hu, D., & Qi, X. (2020). Quantifying drp1-mediated mitochondrial fission by immunostaining in fixed cells. In Methods in Molecular Biology (Vol. 2159, pp. 197–204). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0676-6_15
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