Electromorphic characterization and description of conserved epitopes of the lipooligosaccharides of group A Neisseria meningitidis

Abstract

We studied the lipooligosaccharides (LOS) of 28 group A Neisseria meningitidis of epidemiologically diverse origins to investigate whether each of the LOS serotypes found in serogroup A could be identified physically as wellas antigenically. Using a dot blot assay with LOS-specific monoclonal antibodies (MAbs), we identified four epitopes that were serotype specific. The LOS from strains of each serotype were electromorphically and antigenically distinct when analyzed by silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The LOS of L8 strains contained a 3,600-M(r) component that bound the L8 MAb. The LOS of L9 strains contained two major components of 4,500 and 4,200 M(r). They bound the L9 MAb to the large component. The LOS of L10 strains had a single major component of 4,000 M(r) that bound the L10 MAb. The LOS of L11 strains contained a major 3,600-M(r) component that could not be distinguished from the 3,600-M(r) LOS of L8 strains by SDS-PAGE but that bound the L11 MAb. LOS of group A strains contained a highly conserved epitope in addition to a serotype-specific epitope. This was identified by a MAb that bound to all the strains on dot-blots and to multiple LOS components of various M(r)s on immunoblots. We conclude that the LOS which bear the L9, L10, and L11 determinants are physically distinct and can be identified by SDS-PAGE or MAb binding or both. L8 and L11 are both borne on a 3.6-kilodalton LOS and can only be distinguished serologically.