Fractionation of Saccharomyces cerevisiae cell populations by centrifugal elutriation

Abstract

An exponential population of S. cerevisiae cells was fractionated by centrifugal elutriation, using water as the elutriating liquid. Evidence that the population had been fractionated according to age in the cell cycle was obtained by examining the fractions for their size distribution, their microscopic appearance after Giemsa staining, and their ability to initiate synchronous growth.