Morphological and physiological responses to drought stress of carob trees in Mediterranean ecosystems

Abstract

The greatest failure rate of reforestation programs is basically related to water deficit, especially at the seedling stage. Therefore, the main objective of this work is to investigate the responses of three accessions of carob trees (Ceratonia siliqua L.) with 2-year-old from different climate regions to drought generated by four water treatments: Tc (250 mm), T1 (180 mm), T2 (100 mm), and T3 (50 mm). The first accession (A1) comes from the protected national park of Ichkeul in northern Tunisia. This zone belongs to the bioclimatic sub-humid stage. The second accession (A2) comes from Melloulech, located in the center-east of Tunisia, belonging to the bioclimatic semi-arid stage. The third accession (A3) comes from the mountain of Matmata, located in the south of Tunisia, belonging to the bioclimatic hyper-arid stage. The experiment was undertaken in a greenhouse. Gaz exchange indices (net photosynthesis (A), stomatal conductance (g s), transpiration rate (E), and internal CO2 concentration (C i)) were determined. Predawn (Ψ pd) and midday (Ψ md) leaf water potentials, relative soil water content (SWC), and morphological parameters (plant height (H), number of leaves (NL), number of leaflets (Nl), and number of branches (NB)) were estimated. The results showed that significant differences (P<0.001) were found between physiological and morphological parameters of each accession. The highest growth potential was recorded for Tc treatment in both accessions A1 and A2. Significant decreases in g s, E, C i, and SWC were recorded with the increases in water stress applied from treatment T1 to T3. Positive and significant correlations were found between SWC and Ψ pd for all studied accessions. and Ψ md decreased as water stress increased, ranging from −0.96 to −1.50 MPa at sunrise and from −1.94 to −2.83 MPa at midday, respectively, under control and T3 treatments. C. siliqua accessions responded to drought through exhibiting significant changes in their physiological and morphological behavior. Both accessions A1 and A2 showed greater drought tolerance than accession A3. These seedlings exhibit different adaptive mechanisms such as stress avoidance, which are aimed at reducing transpiration, limiting leaf growth, and increasing root growth to exploit more soil water. Therefore, C. siliqua can be recommended for the ecological restoration in Mediterranean ecosystems.