The majority of proteins in eukaryotic cells are subject to amino-terminal (Nt) acetylation. Recombinant protein expressed using prokaryotic expression systems such as E. coli would not normally be Nt-acetylated as these cells lack the appropriate N-α-terminal acetylation complex. Here we describe a simple protocol that allows the recombinant expression and purification of Nt-acetylated alpha-synuclein (aS) from E. coli.
CITATION STYLE
Eastwood, T. A., & Mulvihill, D. P. (2019). Recombinant expression and purification of N-acetylated alpha-synuclein. In Methods in Molecular Biology (Vol. 1948, pp. 113–121). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9124-2_10
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