Effect of H64V mutation on the dynamical properties of human neuroglobin: A simulation study

Abstract

In this work, we present a classical molecular dynamical simulation of human neuroglobin (Ngb) proteins with and without mutation at the distal position. Our aim is to investigate the role of the distal residue in the stability of Ngb. The simulation has been performed using Gromacs software with Gromos96 force field. We designed a mutant Ngb by mutating histidine His64 residue to valine residue. The results showed that, the mutant H64V would lead to the less stability in the inner structure of the proteins. Moreover, the mutation strongly affects the properties of the heme group. Obvious changes in the high-order structure of the mutant protein can also be observed.