X-ray crystallography is a potent and meanwhile fast technique to obtain detailed structural information of S100 proteins in their apo or metal ion-loaded state. S100 proteins crystallize in the absence or presence of Ca2+ and Zn2+ and the obtained crystals often diffract to high resolution yielding information on the ion-binding sites, conformation, and target interaction sites of the proteins. Here, I describe a general scheme to isolate and crystallize S100 proteins and the analysis of protein crystals using a modern synchrotron source.
CITATION STYLE
Fritz, G. (2013). X-ray structural analysis of S100 proteins. In Methods in Molecular Biology (Vol. 963, pp. 87–97). Humana Press Inc. https://doi.org/10.1007/978-1-62703-230-8_6
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