Effect of hydro-alcoholic root extract of Plumbago zeylanica L alone and it's combination with aqueous leaf extract Camellia sinensis on rotenone induced parkinsonism

Abstract

The aim of this study was to investigate the anti parkinsonism activity of hydro alcoholic root extract of Plumbago zeylanica L. (PZE) alone and its combination with Aqueous Extract of Camellia sinensis (AECS) leaves in Rotenone induced model. Parkinsonism was induced by administration of Rotenone (12 mg/kg, p.o). The effect of PZE (100 mg/kg) and its synergistic effect with AECS were assessed by the ex vivo antioxidant assays by measuring the various enzymes in the striatum region of brain such as Glutathione, Lipid peroxidation, Catalase and Superoxide dismutase. The various behavioral parameters analyzed were rearing, self-grooming, ambulation activity using open field apparatus and muscle rigidity using bar test. The administration of rotenone produced motor dysfunctions like catalepsy and muscle rigidity along with a reduction in locomotor activity. Oxidative stress in the brain was evident from an increase in the level of TBARS and decrease in the levels of catalase, SOD and GSH. Pretreatment with PZE alone and its combination with AECS resulted in a significant (p < 0.001) decrease in catalepsy and muscle rigidity along with a significant (p < 0.001) increase in locomotion as compared to the rotenone-treated group. The reduction in the TBARS level and an increase in the GSH, SOD and CAT levels indicate the reduction in oxidative stress in the brain of animals. It was concluded that P. zeylanica alone and its combination with C. sinensis have a protective effect against rotenone induced Parkinson disease, while bi-herbal extracts exhibited more significant activity than single administration. Hence may offer a safer therapeutic approach to the treatment of Parkinson disease.