Regeneration in Alfalfa Tissue Culture

Abstract

The composition of phosphos-containing compouds of intact lyophilized alfalfa tissue has been determned, in part, by soLid-state 31p nuclear magnetic resonance. The tissue (Mcdicago sativa L, cv Regen S; and some of its crosses) was grown in culture under both nonregeneating and regenerating conditions, the latter enhnced by the addition of specific amino acids. Analysis of the 31P nouclear m etic resonance spectra shows that regeneration is favored when metabolism occurs without the production of a low average intracelular pH.