INTERRELATION AMONG THE SERUM LEUKOTRIENE B4 LEVEL, ARACHIDONATE 5-LIPOXYGENASE ACTIVATING PROTEIN GENE SG13S89G/A POLYMORPHISM AND RISK OF ACUTE MYOCARDIAL INFARCTION

Abstract

Objectives: To investigate the interrelation among the serum leukotriene (LT) B4 level, the arachidonate 5-lipoxygenase activating protein (ALOX5AP) gene SG13S89G/A polymorphism and the risk of acute myocardial infarction (AMI) in the Chinese Han population of Changzhou region. Methods: The polymorphism in the ALOX5AP gene SG13S89G/A was genotyped by PCR and restriction fragment length polymorphism analysis and the serum LTB4 level (M/IQR) was measured by ELISA in 262 patients with AMI (AMI group) and 132 subjects with chest pain who were free from coronary heart disease by coronarography (control group). Results: As compared with those in control group, there were no significant differences in (AA+GA) and GG genetype (7.58% vs 4.96% and 92.42% vs 95.04%, respectively), and A allele frequencie (4.17% vs 2.67%) of ALOX5AP gene SG13S89G/A locus in AMI group (all the p value >0.05). Multivariable logistic regression analysis showed that there was no significant association between ALOX5AP gene SG13S89G/A polymorphism and the risk of AMI. The serum LTB4 level in AMI group was significantly higher than the one in control group (477.97/370.52 pg/ml vs 200.57/236.65 pg/ml, p<0.001). Multivariable logistic regression analysis show that the serum LTB4 level was significantly correlated with the risk of AMI. No significant difference in serum LTB4 levels was found between (AA+GA) and GG genetype in AMI group or control group (all the p value>0.05). Conclusions: The serum LTB4 level is significantly increased in patients with AMI; the ALOX5AP gene SG13S114G/A polymorphism may not be correlated to the risk of AMI and not influence the serum LTB4 level in Chinese Han population of Changzhou region.